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Pilar de la Peña Cortines

Name: de la Peña Cortines, Pilar Dra. Pilar de la Peña Cortines
Citizenship: Spanish
Institution: University of Oviedo
Center: Department of Biochemistry and Molecular Biology
Address: c/Fernando Bongera s/n
Edif. Santiago Gascón. Campus del Cristo B. E-33006.
Oviedo. Spain
Phone: + 34 98-5104211
Fax:
+ 34 98-5103157
pdelapena@uniovi.es







Research

1. Hormonal regulation of ERG channel by G-protein coupled receptors.

- Identification of molecular components of the biochemical pathways involved in hormonal regulation of ERG channels by blockade of their expression or functionality.

- Comparison of mechanisms for hormonal regulation of ERG channels either in heterologous expression systems or in cells in which receptors and channels are endogenously expressed.

- Advancing the knowledge of h-ERG structural requirements for hormonal regulation of the channel.

2.- Analysis of structural architecture and conformational rearrangements of h-ERG channels.

- Generation of fluorescent channel collections randomly labelled with GFP variants using a transposon-based labelling approach.

- Analysis of the oligomeric structure of h-ERG measuring by FRET the distances between individual subunits tagged with CFP and YFP along the channel sequence.

- Analysis by FRET/TIRF and by disulfide bond formation between engineered cysteines pairs of h-ERG conformational rearrangements associated with channel function.

- Use of the collections of h-ERG CFP/VFP fusion proteins obtained by in vitro transposition to introduce new fluorophores able to improve resolution of FRET measurements.


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