SICI database
Login
 

View TABLE: SICI database   Printer Friendly

Back to List 

Date
10/12/2011 11:41:46
Group number
PI name
Felipe, A.
Channel family
voltage-dependent potassium channel
Channel name
Kv1.3, KCNA3
Channel subunit
alpa; beta
UNIPROT
Relevant publication (1)
621.Regulation of a major cloned voltage-gated K+ channel from human T lymphocytes.
Relevant publication (2)
Confirmation of the assignment of the gene encoding Kv1.3, a voltage-gated potassium channel (KCNA3) to the proximal short arm of human chromosome 1.
Relevant publication (3)
Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain.
CD
FTIR
NMR
Absorbance /
Fluorescence
Interaction protein (1)
Kvbeta1 and Kvbeta2
Interaction protein (2)
Kv1.5; Association of Kv1.5 and Kv1.3 contributes to the major voltage-dependent K+ channel in macrophages.
Interaction protein (3)
KCNE4; KCNE4 suppresses Kv1.3 currents by modulating trafficking, surface expression and channel gating.
Interaction protein (4)
sap97 and PSD95
A C-terminal PDZ binding domain modulates the function and localization of Kv1.3 channels; Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17414-9. Beeton et al.
PDB (1)
2R9R Kv1.2/Kv2.1
PDB (2)
2A79 Kv1.2
PDB (3)
Expression tissues
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human fetus. Bielanska J, Hernández-Losa J, Moline T, Somoza R, Ramón Y Cajal S, Condom E, Ferreres JC, Felipe A. Cell Physiol Biochem. 2010;26(2):219-26

KV1.3: a new therapeutic target to control vascular smooth muscle cell proliferation.Jackson WF.Arterioscler Thromb Vasc Biol. 2010 Jun;30(6):1073-4.

Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human cancer. Bielanska J, Hernández-Losa J, Pérez-Verdaguer M, Moline T, Somoza R, Ramón Y Cajal S, Condom E, Ferreres JC, Felipe A. Curr Cancer Drug Targets. 2009 Dec;9(8):904-14.

The voltage-gated potassium channel subunit, Kv1.3, is expressed in epithelia.Grunnet M, Rasmussen HB, Hay-Schmidt A, Klaerke DA. Biochim Biophys Acta. 2003 Sep 22;1616(1):85-94.

Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain. Swanson R, Marshall J, Smith JS, Williams JB, Boyle MB, Folander K, Luneau CJ, Antanavage J, Oliva C, Buhrow SA, et al.Neuron. 1990 Jun;4(6):929-39.
Expression cell lines
Immunological identification of the Shaker-related Kv1.3 potassium channel protein in T and B lymphocytes, and detection of related proteins in files and yeast.Spencer RH, Chandy KG, Gutman GA.Biochem Biophys Res Commun. 1993 Feb 26;191(1):201-6.

Differential voltage-dependent K+ channel responses during proliferation and activation in macrophages. Vicente R, Escalada A, Coma M, Fuster G, Sánchez-Tilló E, López-Iglesias C, Soler C, Solsona C, Celada A, Felipe A.
J Biol Chem. 2003 Nov 21;278(47):46307-20.

Microglia Kv1.3 channels contribute to their ability to kill neurons. Fordyce CB, Jagasia R, Zhu X, Schlichter LC. J Neurosci. 2005 Aug 3;25(31):7139-49.
Expression plasmids
pCDNA3
pYFP
pCFP
pBluescript
Level of expression
No applicable
Channel quaternary
tetramer
Agonists
No applicable
Smile agonist
Antagonists and blockers
Margatoxin
Psora-4
charybdotoxin
dendrotoxin, 4-AP
Smile antagonist
Structure-function
relationship
Exp Cell Res. 2011 Oct 1;317(16):2333-41. Epub 2011 Jun 25. A C-terminal PDZ binding domain modulates the function and localization of Kv1.3 channels. Doczi MA, Damon DH, Morielli AD.
Molecular determinant assembly
Amino acids at the N-terminal region influence pentameric assembly (Castillo, M., et al.(2009) Role of the N-terminal alpha-helix in biogenesis of alpha7 nicotinic receptors. J. Neurochem. 108, 1399-1409. 92; Criado, M., et al.(2010) The loop between beta-strands beta2 and beta3 and its interaction with the N-terminal alpha-helix is essential for biogenesis of alpha7 nicotinic receptors. J. Neurochem. 112, 103-111).

See General Review:Tsetlin, V., et al. (2011) Assembly of nicotinic and other Cys-loop receptors. J. Neurochem. 116, 734–741.Amino acids at the N-terminal region influence pentameric assembly (Castillo, M., et al.(2009) Role of the N-terminal alpha-helix in biogenesis of alpha7 nicotinic receptors. J. Neurochem. 108, 1399-1409. 92; Criado, M., et al.(2010) The loop between beta-strands beta2 and beta3 and its interaction with the N-terminal alpha-helix is essential for biogenesis of alpha7 nicotinic receptors. J. Neurochem. 112, 103-111).

See General Review:Tsetlin, V., et al. (2011) Assembly of nicotinic and other Cys-loop receptors. J. Neurochem. 116, 734–741.
Molecular determinant clustering
Evidence for interaction between transmembrane segments in assembly of Kv1.3. Sheng Z, Skach W, Santarelli V, Deutsch C. Biochemistry. 1997 Dec 9;36(49):15501-13.
Molecular determinant trafficking
Exp Cell Res. 2011 Oct 1;317(16):2333-41. Epub 2011 Jun 25. A C-terminal PDZ binding domain modulates the function and localization of Kv1.3 channels. Doczi MA, Damon DH, Morielli AD.
Molecular determinant binding
Topology of the pore-region of a K+ channel revealed by the NMR-derived structures of scorpion toxins. Aiyar J, Withka JM, Rizzi JP, Singleton DH, Andrews GC, Lin W, Boyd J, Hanson DC, Simon M, Dethlefs B, et al. Neuron. 1995 Nov;15(5):1169-81.

Chemical synthesis and structure-function studies of margatoxin, a potent inhibitor of voltage-dependent potassium channel in human T lymphocytes.
Bednarek MA, Bugianesi RM, Leonard RJ, Felix JP. Biochem Biophys Res Commun. 1994 Jan 28;198(2):619-25.

Purification, characterization, and biosynthesis of margatoxin, a component of Centruroides margaritatus venom that selectively inhibits voltage-dependent potassium channels. Garcia-Calvo M, Leonard RJ, Novick J, Stevens SP, Schmalhofer W, Kaczorowski GJ, Garcia ML. J Biol Chem. 1993 Sep 5;268(25):18866-74.
Mutants constructs
Kv1.3 constructs in all variants of GFP (i.e. YFP, GFP, CFP, Apple, cherry, cerulean, venus
Many mutants and quimeras (in house)
Antibodies
http://www.labome.com/gene/human/Kv1-3-antibody.html (for a full search of Kv1.3 antibodies)

Alomone ltd. (brand specialised in channel antibodies). Alomone Labs: Molecular tools for the Life Science Community (www.alomone.com)
Diseases
Autoimmune diseases:
Ion channels in T lymphocytes: an update on facts, mechanisms and therapeutic targeting in autoimmune diseases. Varga Z, Hajdu P, Panyi G.Immunol Lett. 2010 May 4;130(1-2):19-25. Epub 2009 Dec 21. Review.
Kv1.3 potassium channels as a therapeutic target in multiple sclerosis. Rangaraju S, Chi V, Pennington MW, Chandy KG. Expert Opin Ther Targets. 2009 Aug;13(8):909-24. Review; Targeting effector memory T-cells with Kv1.3 blockers. Wulff H, Pennington M. Curr Opin Drug Discov Devel. 2007 Jul;10(4):438-45. Review.

Obesity and type II diabetes:
Kv1.3: a potential pharmacological target for diabetes. Choi BH, Hahn SJ.Acta Pharmacol Sin. 2010 Sep;31(9):1031-5. Epub 2010 Aug 16. Review; A new variant in the human Kv1.3 gene is associated with low insulin sensitivity and impaired glucose tolerance. Tschritter O, Machicao F, Stefan N, Schäfer S, Weigert C, Staiger H, Spieth C, Häring HU, Fritsche A. J Clin Endocrinol Metab. 2006 Feb;91(2):654-8. Epub 2005 Nov 29;
Kv1.3 potassium channel blockade as an approach to insulin resistance. Desir GV. Expert Opin Ther Targets. 2005 Jun;9(3):571-9. Review.

Neointima hyperplasia (hypertension):
KV1.3: a new therapeutic target to control vascular smooth muscle cell proliferation. Jackson WF. Arterioscler Thromb Vasc Biol. 2010 Jun;30(6):1073-4; Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells. Cidad P, Moreno-Domínguez A, Novensá L, Roqué M, Barquín L, Heras M, Pérez-García MT, López-López JR. Arterioscler Thromb Vasc Biol. 2010 Jun;30(6):1203-11.


Cancer:
Voltage-dependent potassium channels Kv1.3 and Kv1.5 in human cancer.
Bielanska J, Hernández-Losa J, Pérez-Verdaguer M, Moline T, Somoza R, Ramón Y Cajal S, Condom E, Ferreres JC, Felipe A. Curr Cancer Drug Targets. 2009 Dec;9(8):904-14.
Kv1.3 voltage-gated K(+) channel subunit as a potential diagnostic marker and therapeutic target for breast cancer. Jang SH, Kang KS, Ryu PD, Lee SY.BMB Rep. 2009 Aug 31;42(8):535-9.
Reduced Kv1.3 potassium channel expression in human prostate cancer. Abdul M, Hoosein N. J Membr Biol. 2006;214(2):99-102. Epub 2007 Jun 2.

 

  ©SICI Consolider. All rights reserved.